主要从事工业微生物抗逆性和鲁棒性的系统改造，采用系统生物学和合成生物学的方法，在提升工业菌株的鲁棒性、抗热、抗酸碱和抗氧化性，提高菌株对发酵环境的智能响应能力，提高外源基因线路与底盘细胞适配性，以及酵母基因组代谢网络模型与抗逆网络模型构建等方面进行研究，在Metab. Eng.、ACS Energy Let.、Biosens. Bioelectron.、Bioresour. Technol.等SCI期刊上发表论文三十余篇，公开和授权发明专利8项，改造的菌株在企业实现了技术转化，负责或参与科技部、国家自然科学基金委员会以及地方基金等项目11项。
l 教育与工作经历
2005年9月~ 2009年6月 天津大学 生物工程 学士
2009年9月~ 2012年6月 天津大学 生物化工 硕士
2012年7月~ 2013年8月 天津大学 科研助理
2013年9月~ 2017年6月 天津大学 生物化工 博士
2017年10月~ 2020年11月 北京理工大学 博士后
2020年12月~ 2022年12月 清华大学化工系 博士后
2023年3月~ 至今 清华大学化工系 助理研究员
l 研究领域
工业微生物的抗逆设计与工程应用、高版本鲁棒性底盘细胞设计、可再生资源生物转化
l 代表性论文/专利/专著
1. Liu X#, Qin L#, Yu J, Sun W, Xu J, Li C*. Real-time monitoring of subcellular states with genetically encoded redox biosensor system (RBS) in yeast cell factories. Biosensors and Bioelectronics 2023, 222:114988.
2. Qin L, Liu X, Xu K, Li C*. Mining and design of biosensors for engineering microbial cell factory. Current Opinions in Biotechnology 2022, 75:102694.
3. Lv X, Xue H, Qin L*, Li C*. Transporter engineering in microbial cell factory boosts biomanufacturing capacity. BioDesign Research 2022, 9871087.
4. Gao X, Xu K, Ahmad N, Qin L*, Li C. Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance. Biotechnology Journal 2021, 24:e2100151.
5. Qin L, Dong S, Yu J, Ning X, Xu K, Zhang SJ, Xu L, Li BZ, Li J, Yuan YJ, Li C*. Stress-driven dynamic regulation of multiple tolerance genes improves robustness and productive capacity of Saccharomyces cerevisiae in industrial lignocellulose fermentation. Metabolic Engineering 2020, 61:160-170.
6. Xu K#, Qin L#, Bai W, Wang X, Li F, Ren S, Gao X, Chen B, Tong Y, Li J, Li BZ, Yuan YJ, Li C*. Multilevel defense system (MDS) relieves multiple stresses for economically boosting ethanol production of industrial Saccharomyces cerevisiae. ACS Energy Letters 2020, 5:572-582.
7. Huangfu J1, Kim HS1, Xu K, Ning X, Qin L*, Li J*, Li C. Omics analysis reveals the mechanism of enhanced recombinant protein production under simulated microgravity. Frontiers in Bioengineering and Biotechnology 2020, 8:30.
8. Qin L#, Zhao X#, Li WC, Zhu JQ, Liu L, Li BZ*, Yuan YJ. Process analysis and optimization of simultaneous saccharification and co‑fermentation of ethylenediamine‑pretreated corn stover for ethanol production. Biotechnology for Biofuels 2018, 11:118.
9. Qin L#, Li X#, Liu L, Zhu JQ, Guan QM, Zhang MT, Li WC, Li BZ*, Yuan YJ. Dual effect of soluble materials in pretreated lignocellulose on simultaneous saccharification and co-fermentation process for the bioethanol production. Bioresource Technology 2017, 224:342-8.
10. Qin L#, Li X#, Zhu JQ, Li WC, Xu H, Guan QM, Zhang MT, Li BZ*, Yuan YJ. Optimization of ethylenediamine pretreatment and enzymatic hydrolysis to produce fermentable sugars from corn stover. Industrial Crops and Products 2017, 102:51-7.
11. Qin L, Li WC, Liu L, Zhu JQ, Li X, Li BZ*, Yuan YJ. Inhibition of lignin-derived phenolic compounds to cellulase. Biotechnology for Biofuels 2016, 9:70.
12. Qin L, Liu L, Li WC, Zhu JQ, Li BZ*, Yuan YJ. Evaluation of soluble fraction and enzymatic residual fraction of dilute dry acid, ethylenediamine, and steam explosion pretreated corn stover on the enzymatic hydrolysis of cellulose. Bioresource Technology 2016, 209:172-9.
13. Qin L, Li WC, Zhu JQ, Liang JN, Li BZ*, Yuan YJ. Ethylenediamine pretreatment changes cellulose allomorph and lignin structure of lignocellulose at ambient pressure. Biotechnology for Biofuels 2015, 8:174.
14. Liu ZH#, Qin L#, Li BZ*, Yuan YJ. Physical and chemical characterization of corn stover from leading pretreatment methods and effects on enzymatic hydrolysis. ACS Sustainable Chemistry and Engineering 2015, 3:140-6.
15. Qin L, Liu ZH, Jin M, Li BZ, Yuan YJ*. High temperature aqueous ammonia pretreatment and post-washing enhance the high solids enzymatic hydrolysis of corn stover. Bioresource Technology 2013, 146:504-11.
16. Qin L, Liu ZH, Li BZ, Dale BE, Yuan YJ*. Mass balance and transformation of corn stover by pretreatment with different dilute organic acids. Bioresource Technology 2012, 112:319-26.
17. 秦磊, 俞杰, 宁小钰, 孙文涛, 李春. 合成生物系统构建与绿色生物“智”造. 化工学报, 2020, 71(9): 3979-3994.
18. 元英进；秦磊；李炳志；刘志华，一种提高木质纤维素酶解糖化效率的方法，发明专利，2013.7.31. ZL201210094317.7
19. 元英进；秦磊；李文超；李炳志；朱家庆，一种微生物发酵乙醇的方法，发明专利，2017.12.29. ZL201510095249.X
20. 元英进；秦磊；李文超；李炳志；朱家庆，一种木质纤维素原料预处理的方法及应用，发明专利，2018.5.1. ZL201510095389.7
21. 元英进；秦磊；李文超；李炳志；朱家庆，一种木质纤维素原料预处理的方法及应用，发明专利，2019.5.14. ZL201510095265.9
22. Qin L, Li WC, Zhu JQ, Li BZ*, Yuan YJ. Chapter 1. Hydrolysis of Lignocellulosic Biomass to Sugars. In: Fang Z., Smith, Jr. R., Qi X. (eds) Production of Platform Chemicals from Sustainable Resources. Biofuels and Biorefineries. 2017. Springer Nature Singapore.
