Yu, Huimin, Ph.D. , Professor


Department of Chemical Engineering, Tsinghua University
Tel: 8610-62795492
Email: yuhm@tsinghua.edu.cn


  • 2001, Ph.D. in Biochemical Engineering, Tsinghua University , Beijing , P. R. China
  • 1996, B.S. in Chemical Engineering, Tsinghua University , Beijing , P. R. China

Academic Experience

  • Dec 2012, Professor, Department of Chemical Engineering, Tsinghua University, P.R. China
  • Nov 2006-Sept 2005, Visiting scholar in Department of Chemical Engineering,, Massachusetts Institute of Technology, USA
  • Dec 2003, Associate Professor, Department of Chemical Engineering, Tsinghua University, P.R. China
  • Sept 2001, Assistant Professor, Department of Chemical Engineering, Tsinghua University, P.R. China

Selected Award(s)

  • 2016, Award for Cooperation of Science and Technology in Dongying City, National Science and Technology Board of Dongying, Shandong Province.
  • 2013, Award for Technological Invention (2nd prize), China Petroleum and Chemical Industry Association
  • 2010, Outstanding Young Contributor in Science and Technology, China Petroleum and Chemical Industry Association
  • 2008, New Century Excellent Talents in University, Ministry of Education
  • 2007, Beijing Nova (A), Beijing Municipal Science & Technology Commission
  • 2005, Hua-xin Prominent Scholar Award, Tsinghua University
  • 2003, National Top 100 Ph.D. Theses Awards

Research interests


Industrial biocatalysis and Bio-nanotechnology, particularly interested in:

  • Finding and engineering biocatalysts (enzymes and cells) by frontier biotechnologies and applied bioinformatics
  • Intensifying bioprocess via bio-chemical integrated approaches
  • Developing M13 bacteriophage -mediated bio-nanotechnologies and potential applications


Spotlight publications

  • Song Jiao, Xu Li, Huimin Yu*, Huan Yang, Xue Li and Zhongyao Shen. In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters. Biotechnology and Bioengineering 2017, 114(4): 832–842.
  • Shuai Zhang, Huimin Yu*, Ji Yang, Zhongyao Shen. Design of the nanoarray-pattern Fe-Ni bi-metal nanoparticles@M13 virus for enhanced reduction of p-chloronitrobenzene through micro-electrolysis effect. Environmental Science: Nano, 2017, 4, 876 - 885.
  • Fangyu Cheng, Qianying Gong, Huimin Yu*, Gregory Stephanopoulos*, High-titer biosynthesis of hyaluronic acid by recombinant Corynebacterium glutamicum. Biotechnology Journal, 2016, 11(4), 574-584.
  • Jizhe Sun, Huimin Yu*, Jie Chen, Hui Luo and Zhongyao Shen. Ammonium acrylate biomanufacturing by an engineered Rhodococcus ruber with nitrilase overexpression and double-knockout of nitrile hydratase and amidase. Journal of Industrial Microbiology & Biotechnology. 2016, 43(12):1631-1639.
  • Hui Luo*, Linlin Zhu, Yanhong Chang, Xiuhong Liu, Zijia Liu, Hongxu Sun, Xi Li, Huimin Yu, Zhongyao Shen. Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process. Bioresource Technology. 2016, 223:157-165.
  • Jiahui Li, Yang Chen, Mingzhao Guo, Yujun Wang*, Jianhong Xu, Guangsheng Luo, Huimin Yu. Using microchannels to visually investigate the formation and dissolution of acrylonitrile droplets in a bio-hydration system. Chemical Engineering Science, 2016, 152: 239-247.
  • Yuxuan Tian, Jie Chen, Huimin Yu* and Zhongyao Shen, Overexpression of the Escherichia coli chaperones GroEL-GroES in Rhodococcus ruber improves the activity and stability of cell catalysts harboring a nitrile hydratase, J Microbiol Biotechnol, 2016, 26(2): 337–346.
  • Yanmei Wei, Hui Luo*, Yanhong Chang, Huimin Yu and Zhongyao Shen. Reversible immobilization of cephalosporin C acylase on epoxy supports coated with polyethyleneimine. Biocatalysis and Biotransformation, 2016, 33(5-6): 250-259.
  • Huan Yang, Huimin Yu* and Zhongyao Shen, A Novel High-Throughput and Quantitative Method Based on Visible Color Shifts for Screening Bacillus subtilis THY-15 for Surfactin Production, Journal of Industrial Microbiology & Biotechnology, 2015, 42(8): 1139-1147
  • Huan Yang, Xu Li, Xue Li, Huimin Yu* and Zhongyao Shen. Identification of Lipopeptide Isoforms by Tandem MALDI-TOF-MS/MS Based on the Simultaneous Purification of Iturin, Fengycin and Surfactin by RP-HPLC. Analytical and Bioanalytical Chemistry. 2015, 407(9): 2529-2542.
  • Xu Li, Huan Yang, Donglai Zhang, Xue Li, Huimin Yu* & Zhongyao Shen. Overexpression of specific proton motive force-dependent transporters facilitate the export of surfactin in Bacillus subtilis. Journal of Industrial Microbiology & Biotechnology: 2015, 42(1), 93-103.
  • Shuai Zhang, Nakano Kazuhiko, Shu-liang Zhang and Hui-min Yu*. Synthesis of Dispersive Iron or Iron-silver Nanoparticles on Engineered Capsid pVIII of M13 Virus with Electronegative Terminal Peptides. J Nanopart Res (2015) 17(10):417.
  • Li, Jiahui; Chen, Jie; Wang, Yujun; Luo, Guangsheng, Yu Huimin. Hydration of acrylonitrile to produce acrylamide using biocatalyst in a membrane dispersion microreactor Bioresource Technology, 169, 2014, 416-420.
  • Chen Jie, Jia Xu, Yu Huimin*, Luo Hui, Shen Zhongyao. Catalytic kinetics of recombinant nitrile hydratase coupling terminal salt bridge and point-mutation. CIESC Journal. 2014, 65 (7): 2821-2828.
  • Jing Zhang, Huimin Yu*, Ying Wang, Hui Luo and Zhongyao Shen. Determination of the second autoproteolytic cleavage site of cephalosporin C acylase and the effect of deleting its flanking residues in the α-C-terminal region. Journal of Biotechnology 184 (2014) 138–145.
  • Shuai Zhang, Kazuhiko Nakano, Huimin Yu* and Zhongyao Shen. Bio-nano complexes of ZVFeNPs/Fe-s-M13 and Cd (II)/Cd-s-M13 accelerate Cd (II) reduction by FeNPs through dual dispersing and separate deposition. Materials Research Express. 1 (2014) 015043, 13 pages.
  • Jian Zhang, Zi-Ting Wang, Hui-Min Yu* and Yuchao Ma*. Paenibacillus catalpae sp. nov., isolated from the rhizosphere soil of Catalpa speciosa. Int J Syst Evol Microbiol 2013, 63:1776-1781.
  • Fangyu Cheng, Cheng Tang, Huan Yang, Huimin Yu*, Yu Chen and Zhongyao Shen. Characterization of a blend-biosurfactant of glycolipid and lipopeptide produced by Bacillus subtilis TU2 isolated from underground oil-extraction wastewater. J Microbiol Biotechnol. 2013; 23(3):390-396.
  • Jie Chen, Huimin Yu*, Changchun Liu, Jie Liu and Zhongyao Shen. Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions. Journal of Biotechnology 164 (2012) 354–362
  • Yuchao Ma*, Huimin Yu* and Zhongyao Shen. Engineering Rhodococcus cell-catalysts for tolerance improvement by mutant sigma factor and plasmid active partition. Journal of Industrial Microbiology & Biotechnology. 2012, 39: 1421-1430.
  • Jian Zhang, Yuchao Ma* and Huimin Yu*. Arthrobacter cupressi sp. nov., a novel actinomycete isolated from the rhizosphere soil of Cupressus sempervirens. International Journal of Systematic and Evolutionary Microbiology, 2012, 62, 2731–2736.
  • Jian Zhang, Yuchao Ma* and Huimin Yu*. Nocardioides lianchengensis sp. nov., a novel actinomycete isolated from soil from Liancheng county, Fujian province, China. Int J Syst Evol Microbiol. 2012, 62, 2698–2702.
  • Ying Wang, Huimin Yu*, Jing Zhang, Hui Luo and Zhongyao Shen. Double knockout of β-lactamase and cephalosporin acetyl esterase genes from Escherichia coli reduces cephalosporin C decomposition. Journal of Bioscience and Bioengineering, 2012, 113(6), 737-741
  • Wang, Y., Yu, H., Song, W., An M., Zhang J., Luo, H., and Shen Z.: Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel. Journal of Bioscience and Bioengineering, 2012, 113(1):36–41.
  • Luo Hui, Fan Lu, Chang Yanhong, Ma Jinwei, Yu Huimin, Shen Zhongyao. Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli. Applied Biochemistry and Biotechnology, 2010, 160(2): 393-400.
  • Yuchao Ma, Huimin Yu*, Wenyu Pan, Changchun Liu and Zhongyao Shen. Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of the amiE-inactivation mutant. Bioresource technology, 2010, 101(1): 285-291.
  • Tao Ling, Jing Zhu, Huimin Yuand Lin Xie. Size Effect on Crystal Morphology of Faceted Face-Centered Cubic Fe Nanoparticles. Journal of Physical Chemistry C. 2009, 113(22): 9450-9453.
  • Daniel Klein-Marcuschamer, Christine Nicole S. Santos, Huimin Yu, and Gregory Stephanopoulos. Mutagenesis of the bacterial RNA polymerase alpha subunit for improving complex phenotypes. Appl. Environ. Microbiol. 2009, 75(9):2705-2711.
  • Tao Ling, Lin Xie, Jing Zhu, Huimin Yu, et al. Icosahedral Face-Centered Cubic Fe Nanoparticles: Facile Synthesis and Characterization with Aberration-Corrected TEM. Nano letters. 2009, 9 (4), pp 1572–1576.
  • Tao Ling, Huimin Yu, Xiaohua Liu, Zhongyao Shen, Jing Zhu. Fivefold Twinned Nanorods of FCC Fe: Synthesis and Characterization. Crystal Growth & Design, 2008, 8 (12), 4340–4342.
  • Jie Liu, Huimin Yu*, Zhongyao Shen. Insights into thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation. Journal of Molecular Graphics and Modelling, 2008, 27(4): 529-535.
  • Huimin Yu*, Jie Liu, Zhongyao Shen. Modelling catalytic mechanism of nitrile hydratase by semi-empirical quantum mechanical calculation. Journal of Molecular Graphics and Modelling, 2008, 27(4): 522-528.
  • Huimin Yu, Keith Tyo, H. Alper, D. Marcuschamer, Gregory Stephanopoulos*. A High Throughput Screen for Hyaluronic Acid Accumulation in Recombinant Escherichia coli Transformed by Libraries of Engineered Sigma Factors.  Biotechnology and Bioengineering. 2008, 101 (4): 788-796.
  • Huimin Yu* and Gregory Stephanopoulos*. Metabolic Engineering of Escherichia coli for biosynthesis of hyaluronic acid. Metabolic Engineering. 2008, 10(1): 24-32.
  • Tao Ling, Huimin Yu, Zhongyao Shen, Hui Wang, Jing Zhu. Virus-mediated FCC Iron Nanoparticle Induced Synthesis of Uranium Dioxide Nanocrystal. Nanotechnology, 2008, 19(11):115608 (6pp).