Zhanglin Lin, Bairen Program Professor of Biological Engineering
Tel: +86 (10) 6279-4403; Fax: +86 (10) 6277-0304
Email: zhanglinlin@mail.tsinghua.edu.cn

B.S., Chemical Engineering (with a minor in economics), Tsinghua University (1990)
M.S., Biochemical Engineering, University of Maryland, Baltimore (1993)
Ph.D., Molecular and Cell Biology, University of Maryland, College Park & Center for Advanced Research in Biotechnology (CARB), Rockville, MD (1996)
Postdoctoral, Division of Chemistry & Chemical Engineering, Caltech (1996-1999)


Research in Professor Lin¡¯s lab focuses on addressing fundamental technical problems related to molecular evolution of proteins, and on engineering proteins related to various bioprocesses. At present, there are three specific areas of research that are being pursued: (1) development of tools for directed evolution, for example, a high thorough-put autolysis scheme for E. coli cells; (2) evolution of useful biocatalysts and pathways, which includes engineering of enzymes and regulatory proteins related to biorefinery and for production of fine chemicals; (3) dissection and functional analysis of enzymes and viral proteins. Research in this lab has been supported by the national key basic research (973) program, the national high-tech (863) program, NSFC, and industrial grants. Prof. Lin also has been teaching an undergraduate course ¡°principles and applications of genetic engineering.¡±

Selected Publications:

      1. Y. Chen, S. Li, T. Chen, H. Hu, and Z. Lin (2009) Random Dissection to Select for Protein Split Sites and Its Application in Protein Fragment Complementation. Protein Science 18, 399-409.
      2. Z. Lin, S. Li, and Y. Chen (2009). Identification of Viral Peptide Fragments for Vaccine Development. In Viral Applications of the Green Fluorescent Protein, Methods in Molecular Biology Series (B.W. Hicks, ed.), in press.
      3. Z. Cai, W. Xu, R. Xue, and Z. Lin (2008) Facile, Reagentless and in situ Release of E. coli Intracellular Enzymes by Heat-inducible Autolytic Vector for High-throughput Screening. Protein Engineering, Design and Selection 21, 681-687 (cover illustration).
      4. L. Liang, J. Zhang, Z. Lin (2007). Altering Coenzyme Specificity of Pichia stipitis Xylose Reductase by the Semi-ratioal Approach CASTing. Microbial Cell Factories 6:36.
      5. L. Xu, S. Li, C. Ren, Z. Cai, and Z. Lin (2006). Heat-inducible Autolytic Vector for High Throughput Screening. Biotechniques 41: 319-323.
      6. H. Joo, Z. Lin and F. H. Arnold. (1999). Laboratory Evolution of Peroxide-mediated Cytochrome P450 Hydroxylation. Nature 399, 670-673.
      7. Z. Lin, and E. Eisenstein (1996). Nucleotide Binding-promoted Changes Release a Non-native Polypeptide from the Escherichia coli Chaperonin GroEL. Proc. Natl. Acad. Sci. USA 93, 1977-1981.


Most Recent Update£º2009-3-1