陈 振

副教授(特别研究员),博士生导师

 

研究方向:合成生物学与代谢工程、医药及精细化学品的绿色生物制造

 

办公电话:010-62772130-108

办公地址:清华大学英士楼314

电子邮件:zhenchen2013@tsinghua.edu.cn

 

本课题组常年招收博士生、硕士生,欢迎推免和报考!

 

教育经历:

2008/08-2012/06,德国汉堡工业大学,生物过程与生物系统工程研究所,博士

2005/09-2008/06,清华大学、东京工业大学,化学工程/生物工程,硕士

2001/09-2005/06,清华大学,化学工程系,学士

工作经历:

2017/08-至今,清华大学,化学工程系,副教授,特别研究员

2013/08-2017/07,清华大学,化学工程系,助理教授,特别研究员

2012/07-2013/08,德国汉堡工业大学,生物过程与生物系统工程研究所,博士后

获得奖励:

2011年,国家优秀自费留学生奖学金

43届日内瓦国际发明博览会金奖

研究方向:

本实验室一直专注于材料、化学品及生物医药的绿色生物制造,结合蛋白质工程、代谢工程及合成生物学等交叉领域的前沿技术,综合利用工程学、生物学、化学和计算机科学等多学科交叉手段来研究酶及细胞催化过程中的重要基础科学问题及工业化应用技术。发展了非天然途径设计、新功能酶的挖掘与改造、原子经济的产品设计工程等系列新方法,在氨基酸及衍生物、生物基二元醇、精细化学品等重要产品的工业化生产方面取得显著成果。迄今在国内外期刊会议上发表学术论文30多篇,集中发表在本研究领域的顶级期刊如Trends in Biotechnology, Metabolic Engineering, ACS Synthetic Biology等,获得广泛关注。申请专利13项(中国专利8项,欧洲专利5项),研究成果被中粮集团、Metabolic Explorer等国际企业所采用。

 

目前课题组的研究方向包括:(1)基于合成生物学的产品设计工程与应用;(2)重要化学品的生物制造,主要包括营养健康类产品(半胱氨酸等氨基酸产品),生物基材料单体(1,3-丙二醇等二元醇),美容保健类产品(透明质酸、四氢嘧啶等)。与国内外大型企业如中粮集团等有密切的合作。

 

主持科研项目:

1. 国家自然科学基金青年项目,非甘油衍生的全新的1,3-丙二醇生物合成途径的构建与机理研究(主持)。

2. 广东省科技计划项目,平台化合物3-羟基丙酸的生物炼制(主持)。

3. 清华大学自主科研项目,非天然代谢途径重构及其用于生产生物基乙二醇的基础研究(主持)。

4. 国家自然科学基金面上项目:基于序列深度突变扫描高通量改造胞内色氨酸新生肽生物传感器响应特性 (课题骨干)。

5. 国家重点研发计划:生物产业共性技术标准研究重点专项(课题骨干)。

6. 苏州-清华创新引领行动专项:1,3-丙二醇绿色合成生物技术及产业化(副组长)。

7. 企业横向项目:高产1,3-丙二醇的重组谷氨酸棒杆菌的开发(主持)。

 

代表性论文:

W Wu, Y Zhang, J Huang, Y Wu, D Liu, Z Chen*.Discovery of a potentially new subfamily of ELFV dehydrogenases effective for Larginine deamination by enzyme mining. Biotechnology Journal, DOI: 10.1002/biot.201700305.

 

J Huang, Y Wu, W Wu, Y Zhang, D Liu, Z Chen*. Cofactor recycling for co-production of 1, 3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum. Scientific Reports, 2017, 7, 42246.

 

Z Chen*, J Huang, Y Wu, W Wu, Y Zhang, D Liu. Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose. Metabolic engineering, 2017, 39, 151-158.

 

Chen Z, Zeng AP*. Protein engineering approaches to chemical biotechnology. Current Opinion in Biotechnology, 2016, 42, 198-205.

 

Chen Z*, Huang J, Wu Y, Liu DH. Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose. Metabolic engineering, 2016, 33, 12-18.

 

Chen Z*, Sun H, Huang J, Wu Y, Liu DH. Metabolic engineering of Klebsiella pneumoniae for the production of 2-butanone from glucose. PloS one, 2015, 10 (10), e0140508.

 

Chen Z*, Wu Y, Huang J, Liu DH. Metabolic engineering of Klebsiella pneumoniae for the de novo production of 2-butanol as a potential biofuel. Bioresource technology, 2015, 197, 260-265.

 

Chen Z#, Geng F#, Zeng AP*. 2015. Protein design and engineering of a de novo pathway for microbial production of 1,3-propanediol from glucose. Biotechnol Journal, 2015, 10(2):284-289.

 

Chen Z Rappert S Zeng AP*. Rational design of allosteric regulation of homoserine dehydrogenase by a non-natural inhibitor L-Lysine. ACS Synthetic Biology, 2015, 4(2):126-131.

 

Bommareddy RR#, Chen Z#, Rappert S, Zeng AP*. A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Metabolic Engineering, 2014, 25:30-37.

 

Chen Z Bommareddy R, Frank D, Rappert S Zeng AP*. Deregulation of Feedback Inhibition of Phosphoenolpyruvate Carboxylase for Improved Lysine Production in Corynebacterium glutamicum. Applied and Environmental Microbiology, 2014, 80(4):1388-1393.

 

Chen ZJandt U Rappert S Zeng AP*. Protein-Design für die Entwicklung von industriellen Mikroorganismen. BIOspektrum, 2013, 19(1)99-101.

 

Chen, ZZeng AP*. Protein design in systems metabolic engineering for industrial strain development. Biotechnol Journal, 2013, 97(7):2939-2949.

 

Geng F#, Chen Z#, Zheng P, Sun JB, Zeng AP*. Exploring the allosteric mechanism of dihydrodipicolinate synthase by reverse engineering of the allosteric inhibitor binding sites and its application for lysine production. Applied Microbiology and Biotechnology, 2012, 97(5):1963-1971

 

Chen L#, Chen Z#, Sun JB, Zeng AP*. Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli. Applied Microbiology and Biotechnology, 2012, 97(7):2939-2949

 

Chen Z, Meyer WQ, Rappert S, Sun JB, Zeng AP*. Coevolutionary analysis enabled rational deregulation of allosteric enzyme inhibition in Corynebacterium glutamicum for lysine production. Applied and Environmental Microbiology, 2011, 77(29):4352-4360.

 

Chen Z, Rappert S, Sun JB, Zeng AP*. Integrating molecular dynamics and co-evolutionary analysis for reliable target prediction and deregulation of the allosteric inhibition of aspartokinase for amino acid production. Journal of Biotechnology2011154: 248-254.

 

Chen Z, Liu HJ, Liu DH*. Metabolic pathway analysis of 1,3-propanediol production with a genetically modified Klebsiella pneumoniae by overexpressing an endogenous NADPH-dependent alcohol dehydrogenase. Biochemical Engineering Journal, 2011, 54:151-157.

 

Chen Z, Wilmanns M, Zeng AP*. Structural synthetic biotechnology: from molecular structure to predictable design for industrial strain development. Trends in biotechnology 2010, 28 (10): 534-542.

 

Chen Z, Liu H, Zhang J, Liu D*. Elementary mode analysis for the rational design of efficient succinate conversion from glycerol by Escherichia coli. Journal of Biomedicine and Biotechnology, 2010, 2010:518743.

 

Chen Z, Liu H, Liu D*. Regulation of 3-hydroxypropionaldehyde accumulation in Klebsiella pneumoniae by overexpression of dhaT and dhaD genes. Enzyme and Microbial Technology, 2009, 45 (4): 305-309.

 

Chen Z, Liu H, Zhang J, Liu D*. Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions. Process Biochemistry, 2009, 44 (8)862-868.

Zhen Chen; Hongjuan Liu; Jianan Zhang; Dehua Liu. Metabolic pathway analysis: principles, examples and application. Genomics II: Bacteria, Viruses, and Metabolic Pathways),iConcept Press. 2013, ISBN: 978-1-922227-041.

Zhen Chen. Protein Design for Industrial Strain Development: the Case of Amino Acid Production, Shaker Verlag GmbH, 2012, ISBN: 978-3844015546.